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transscript one-step gdna removal and cdna synthesis supermix kit  (Beijing TransGen Biotech)

 
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    Beijing TransGen Biotech transscript one-step gdna removal and cdna synthesis supermix kit
    Transscript One Step Gdna Removal And Cdna Synthesis Supermix Kit, supplied by Beijing TransGen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transscript+one-step+gdna+removal+and+cdna+synthesis+supermix+kit/transscript+one+step+gdna+removal+and+cdna+synthesis+supermix+kit/10__1016_slash_j__postharvbio__2025__113613-63-14-23
    Average 90 stars, based on 1 article reviews
    transscript one-step gdna removal and cdna synthesis supermix kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Knockout of SlCBF1 enhances tomato fruit resistance against Botrytis cinerea after chilling stress treatment
    Article Snippet: C-repeat/dehydration-responsive element binding factor 1 (CBF1) plays a pivotal role in protecting plants from chilling stress.. There is an extensive signal crosstalk between cold and pathogen responses.. However, the role of SlCBF1 in tomato disease resistance in tomato remains poorly understood.

    Article Title: Ethylene perception influences the chilling injury and SlCBF1 gene expression during cold storage of tomato fruit
    Article Snippet: Endogenous and exogenous ethylene were reported to positively regulate the cold tolerance, and mitigate the chilling injury (CI) damage of stored tomato fruit.. However, the involvement of ethylene perception in cold resistance is still unclear and needs further investigation in postharvest fruit.. Here, we used Never ripen (Nr) and wild-type (WT) tomato fruit as inhibitedand normal-ethylene perception models to investigate how inhibiting ethylene perception affected fruit quality and cold responses of stored tomato fruit.

    Article Title: Genomics and transcriptomics reveal β-carotene synthesis mechanism in Dunaliella salina
    Article Snippet: .. Using the RNA from the control and experimental groups on the 5th and 13th days of culture as templates, reverse transcription was performed according to the instructions of the TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix kit(Beijing TransGen Biotech Co., Ltd.) The obtained cDNA was used as a template, and fluorescence quantification detection was performed using the ABI Step One fluorescent quantitative PCR instrument, according to the instructions of the Quanshijin TransStart®TOP Green qPCR SuperMix kit. ..

    Article Title: Synergistic effects of SlCBF1 and ethylene signaling on the maintenance of tomatoes quality during long-term cold storage
    Article Snippet: Refrigeration is commonly employed for postharvest storage of fruit and vegetable; however, its usage can increase the risk of chilling injury (CI).. C-repeat/dehydration-responsive element binding factors (CBFs) have been identified in various plants.. However, the significance of CBFs in preserving fruit quality during cold storage remains understudied, particularly in transgenic fruit.

    Article Title: Slibinin governs high glucose induced autophagy in cardiac myocyte cells via sphingosine kinase 1 pathway
    Article Snippet: As a disorder of the myocardium caused by diabetes mellitus, DCM has become a key health concern in the world.. Autophagy plays an important role in the pathogenesis of DCM.. Sphingosine kinase 1 benefits to cell survival and growth, and regulates the pathogenesis of many diseases, including diabetes and cardiovascular disease.

    Article Title: Effects of histone methylation modification on low temperature seed germination and growth of maize
    Article Snippet: .. We used the reverse transcription of total RNA was perormed by TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix kit from Beijing TransGen Biotechnology Co., LTD. We used PerfectStartTM Green qPCR SuperMix kit from Beijing TransGen Biotechnology Co., LTD and SYBR dye method was used to detect by fluorescence quantitative PCR, the Actin was used as the internal ref-erence primer. ..

    cDNA Synthesis:

    Article Title: Knockout of SlCBF1 enhances tomato fruit resistance against Botrytis cinerea after chilling stress treatment
    Article Snippet: C-repeat/dehydration-responsive element binding factor 1 (CBF1) plays a pivotal role in protecting plants from chilling stress.. There is an extensive signal crosstalk between cold and pathogen responses.. However, the role of SlCBF1 in tomato disease resistance in tomato remains poorly understood.

    Article Title: Postharvest fruit quality of tomatoes influenced by an ethylene signaling component during long-term cold storage.
    Article Snippet: Ethylene production is essential for improving cold resistance of postharvest tomatoes.. However, the role of ethylene signaling pathway in maintaining fruit quality during long-term cold storage remains poorly understood.. Here, we demonstrated that a partial loss of function in ethylene signaling by mutation of Ethylene Response Factor 2 (SlERF2), worsened fruit quality during cold storage, as determined by visual characterization, and physiological analyses of membrane damage and reactive oxygen species metabolism.

    Article Title: Ethylene perception influences the chilling injury and SlCBF1 gene expression during cold storage of tomato fruit
    Article Snippet: Endogenous and exogenous ethylene were reported to positively regulate the cold tolerance, and mitigate the chilling injury (CI) damage of stored tomato fruit.. However, the involvement of ethylene perception in cold resistance is still unclear and needs further investigation in postharvest fruit.. Here, we used Never ripen (Nr) and wild-type (WT) tomato fruit as inhibitedand normal-ethylene perception models to investigate how inhibiting ethylene perception affected fruit quality and cold responses of stored tomato fruit.

    Article Title: Genomics and transcriptomics reveal β-carotene synthesis mechanism in Dunaliella salina
    Article Snippet: .. Using the RNA from the control and experimental groups on the 5th and 13th days of culture as templates, reverse transcription was performed according to the instructions of the TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix kit(Beijing TransGen Biotech Co., Ltd.) The obtained cDNA was used as a template, and fluorescence quantification detection was performed using the ABI Step One fluorescent quantitative PCR instrument, according to the instructions of the Quanshijin TransStart®TOP Green qPCR SuperMix kit. ..

    Article Title: Arginine and cysteine delay postharvest ripening of tomato fruit by regulating ethylene production
    Article Snippet: Ripe fruit, rich in essential nutrients, holds significant economic value.. In this study, we investigate the dynamics of amino acids during the shelf-life of tomato fruit.. To examine their influence on fruit ripening, we treat tomato fruit with either L-arginine (Arg) or L-cysteine (Cys).

    Article Title: Synergistic effects of SlCBF1 and ethylene signaling on the maintenance of tomatoes quality during long-term cold storage
    Article Snippet: Refrigeration is commonly employed for postharvest storage of fruit and vegetable; however, its usage can increase the risk of chilling injury (CI).. C-repeat/dehydration-responsive element binding factors (CBFs) have been identified in various plants.. However, the significance of CBFs in preserving fruit quality during cold storage remains understudied, particularly in transgenic fruit.

    Article Title: Slibinin governs high glucose induced autophagy in cardiac myocyte cells via sphingosine kinase 1 pathway
    Article Snippet: As a disorder of the myocardium caused by diabetes mellitus, DCM has become a key health concern in the world.. Autophagy plays an important role in the pathogenesis of DCM.. Sphingosine kinase 1 benefits to cell survival and growth, and regulates the pathogenesis of many diseases, including diabetes and cardiovascular disease.

    Article Title: Effects of histone methylation modification on low temperature seed germination and growth of maize
    Article Snippet: .. We used the reverse transcription of total RNA was perormed by TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix kit from Beijing TransGen Biotechnology Co., LTD. We used PerfectStartTM Green qPCR SuperMix kit from Beijing TransGen Biotechnology Co., LTD and SYBR dye method was used to detect by fluorescence quantitative PCR, the Actin was used as the internal ref-erence primer. ..

    Synthesized:

    Article Title: Postharvest fruit quality of tomatoes influenced by an ethylene signaling component during long-term cold storage.
    Article Snippet: Ethylene production is essential for improving cold resistance of postharvest tomatoes.. However, the role of ethylene signaling pathway in maintaining fruit quality during long-term cold storage remains poorly understood.. Here, we demonstrated that a partial loss of function in ethylene signaling by mutation of Ethylene Response Factor 2 (SlERF2), worsened fruit quality during cold storage, as determined by visual characterization, and physiological analyses of membrane damage and reactive oxygen species metabolism.

    Article Title: Arginine and cysteine delay postharvest ripening of tomato fruit by regulating ethylene production
    Article Snippet: Ripe fruit, rich in essential nutrients, holds significant economic value.. In this study, we investigate the dynamics of amino acids during the shelf-life of tomato fruit.. To examine their influence on fruit ripening, we treat tomato fruit with either L-arginine (Arg) or L-cysteine (Cys).

    Real-time Polymerase Chain Reaction:

    Article Title: Postharvest fruit quality of tomatoes influenced by an ethylene signaling component during long-term cold storage.
    Article Snippet: Ethylene production is essential for improving cold resistance of postharvest tomatoes.. However, the role of ethylene signaling pathway in maintaining fruit quality during long-term cold storage remains poorly understood.. Here, we demonstrated that a partial loss of function in ethylene signaling by mutation of Ethylene Response Factor 2 (SlERF2), worsened fruit quality during cold storage, as determined by visual characterization, and physiological analyses of membrane damage and reactive oxygen species metabolism.

    Article Title: Genomics and transcriptomics reveal β-carotene synthesis mechanism in Dunaliella salina
    Article Snippet: .. Using the RNA from the control and experimental groups on the 5th and 13th days of culture as templates, reverse transcription was performed according to the instructions of the TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix kit(Beijing TransGen Biotech Co., Ltd.) The obtained cDNA was used as a template, and fluorescence quantification detection was performed using the ABI Step One fluorescent quantitative PCR instrument, according to the instructions of the Quanshijin TransStart®TOP Green qPCR SuperMix kit. ..

    Article Title: Arginine and cysteine delay postharvest ripening of tomato fruit by regulating ethylene production
    Article Snippet: Ripe fruit, rich in essential nutrients, holds significant economic value.. In this study, we investigate the dynamics of amino acids during the shelf-life of tomato fruit.. To examine their influence on fruit ripening, we treat tomato fruit with either L-arginine (Arg) or L-cysteine (Cys).

    Article Title: Effects of histone methylation modification on low temperature seed germination and growth of maize
    Article Snippet: .. We used the reverse transcription of total RNA was perormed by TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix kit from Beijing TransGen Biotechnology Co., LTD. We used PerfectStartTM Green qPCR SuperMix kit from Beijing TransGen Biotechnology Co., LTD and SYBR dye method was used to detect by fluorescence quantitative PCR, the Actin was used as the internal ref-erence primer. ..

    Control:

    Article Title: Genomics and transcriptomics reveal β-carotene synthesis mechanism in Dunaliella salina
    Article Snippet: .. Using the RNA from the control and experimental groups on the 5th and 13th days of culture as templates, reverse transcription was performed according to the instructions of the TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix kit(Beijing TransGen Biotech Co., Ltd.) The obtained cDNA was used as a template, and fluorescence quantification detection was performed using the ABI Step One fluorescent quantitative PCR instrument, according to the instructions of the Quanshijin TransStart®TOP Green qPCR SuperMix kit. ..

    Fluorescence:

    Article Title: Genomics and transcriptomics reveal β-carotene synthesis mechanism in Dunaliella salina
    Article Snippet: .. Using the RNA from the control and experimental groups on the 5th and 13th days of culture as templates, reverse transcription was performed according to the instructions of the TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix kit(Beijing TransGen Biotech Co., Ltd.) The obtained cDNA was used as a template, and fluorescence quantification detection was performed using the ABI Step One fluorescent quantitative PCR instrument, according to the instructions of the Quanshijin TransStart®TOP Green qPCR SuperMix kit. ..

    Article Title: Effects of histone methylation modification on low temperature seed germination and growth of maize
    Article Snippet: .. We used the reverse transcription of total RNA was perormed by TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix kit from Beijing TransGen Biotechnology Co., LTD. We used PerfectStartTM Green qPCR SuperMix kit from Beijing TransGen Biotechnology Co., LTD and SYBR dye method was used to detect by fluorescence quantitative PCR, the Actin was used as the internal ref-erence primer. ..



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